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Due to increased consumption, artificial sweeteners are often present in the environment but their effects on plants are largly unknown. In this research, the effects of four artificial sweeteners on plant stress markers in Triticum aestivum L. were investigated. Wheat seedlings were grown from seeds in soil containing artificial sweeteners (saccharin, sodium cyclamate, sucralose, aspartame) in different concentrations (0, 25, 50, 100 mg kg–1). Plants were irrigated at regular intervals to maintain field capacity moisture and harvested after 15 days of growth. Electrolyte leakage, chlorophyll and carotenoid content, and antioxidant enzyme (superoxide dismutase, peroxidase, catalase) activities were determined in harvested leaves. Comparisons between control samples and test samples were statistically evaluated at a 95% confidence interval to determine significant differences. Overall, significant increases in chlorophyll and carotenoid content, and some antioxidant enzyme activities were observed in wheat plants exposed to artificial
sweeteners in the soil. A significant increase in electrolyte leakage was observed with saccharin and aspartame treatment, indicating that these sweeteners can cause membrane damage in wheat. Chlorophyll a and POX activity were the most sensitive stress parameters in wheat. This study showed the importance of evaluating the potential impact
of anthropogenic pollutants that may be present in treated wastewater and consequently affect plants.


artificial sweeteners wheat electrolyte leakage catalase peroxidase superoxide dismutase

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How to Cite
ELVEREN, M. (2024). Effects of artificial sweeteners on antioxidant enzymes and physiological parameters in Triticum aestivum (Poaceae). Acta Botanica Croatica, 83(1), 69–75.