TY - JOUR AU - Kaya, Ergun AU - Galatali, Selin AU - Souza, Fernanda Vidigal Duarte AU - Santos-Serejo, Janay Almeida PY - 2020/10/01 Y2 - 2024/03/28 TI - Influence of dehydration on cryopreservation of Musa spp. germplasm JF - Acta Botanica Croatica JA - Acta Bot. Croat. VL - 79 IS - 2 SE - Articles DO - 10.37427/botcro-2020-024 UR - https://www.abc.botanic.hr/index.php/abc/article/view/2691 SP - 99-104 AB - <p><span data-contrast="auto">Cryopreservation is an important technique for the long-term storage of economically important plant germplasm. In this study, an efficient protocol was developed for the long-term conservation of seven economically important </span><em><span data-contrast="auto">Musa</span></em><span data-contrast="auto"> taxa: </span><em><span data-contrast="auto">M</span></em><span data-contrast="auto">.</span><em><span data-contrast="auto"> acuminata</span></em><span data-contrast="auto"> Colla ssp. </span><em><span data-contrast="auto">burmannica</span></em><span data-contrast="auto"> N.W. Simmonds, </span><em><span data-contrast="auto">M. acuminata</span></em><span data-contrast="auto"> Colla ssp. </span><em><span data-contrast="auto">zebrina</span></em><span data-contrast="auto"> (Van Houtte) R.E. Nasution, </span><em><span data-contrast="auto">M. balbisiana</span></em><span data-contrast="auto"> Colla, </span><em><span data-contrast="auto">M. basjoo</span></em><span data-contrast="auto"> Sieb., </span><em><span data-contrast="auto">M. ornata</span></em><span data-contrast="auto"> W. Roxburgh (St. Lavender), </span><em><span data-contrast="auto">M. velutina</span></em><span data-contrast="auto"> H. Wendl. et Drude (Velvet Pink Banana), and </span><em><span data-contrast="auto">M</span></em><span data-contrast="auto">.</span><em><span data-contrast="auto"> acuminata</span></em> <span data-contrast="auto">´</span> <em><span data-contrast="auto">balbisiana</span></em><span data-contrast="auto">. The seeds were dehydrated in a sterile laminar flow cabinet for different exposure times and then they were directly immersed in liquid nitrogen. The critical point was to support the initial germination of cryopreserved seeds and this was achieved by the excision of zygotic embryos after liquid nitrogen treatment that allowed the seed germination. The best moisture content for tolerance to cryopreservation ranged from 15.8% (</span><em><span data-contrast="auto">M. acuminata </span></em><span data-contrast="auto">ssp.</span><em><span data-contrast="auto"> zebrina</span></em><span data-contrast="auto">) to 17.1% (</span><em><span data-contrast="auto">M. ornata</span></em><span data-contrast="auto">) and the maximum post-cryopreservation germination rates varied from 86.4% (</span><em><span data-contrast="auto">M. velutina</span></em><span data-contrast="auto">) to 55.0% (</span><em><span data-contrast="auto">M. ornata</span></em><span data-contrast="auto">). All seedlings derived from seeds germinated after cryopreservation were easily rooted and acclimated to greenhouse conditions.</span><span data-ccp-props="{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559739&quot;:200,&quot;335559740&quot;:240}"> </span></p> ER -